Single cells or nuclei from solid tissues in minutes

Explore the Singulator™100

Single cells or nuclei from solid tissues in minutes

Explore the Singulator™100

Solid Tissue Dissociation. Automated.

The bench-top Singulator System and its single-use cartridges enable reproducible, rapid and hands-off tissue dissociations into single-cell or nuclei suspensions. Its ability to perform cold dissociation minimizes the expression of stress-related genes in cells and helps preserve RNA quality in nuclei. Researchers can now easily obtain suspensions of nuclei or high-viability cells for a wide range of single-cell analyses.

Watch this video to learn more about the Singulator 100 System.

Say Goodbye To Manual Tissue Dissociation.

From tissue to single cells or nuclei in minutes with walk-away operation.

Minimal Variability. Multiple Applications.

Ideal for genomics, cell biology and other ‘omics applications, including scRNA-Seq, snRNA-Seq, ATAC-Seq, CITE-Seq, FACS, and immuno-oncology. S2 Genomics provides a selection of pre-set protocols and pre-formulated reagents for cell isolations for an expanding set of mouse, rat, and human tissues, including tumors. See the wide range of tissues and organisms demonstrated on the Singulator 100 System here.

Minimal Variability. Multiple Applications.

Fast. High Yield. High Viability.

Process fresh, frozen and OCT tissues.

	TISSUE TYPE	Process Time	Yield*	Viability*
Cells	Fresh	20-60 minutes	10,000 to >500,000/mg	70-95%
Nuclei	Fresh, Frozen, OCT	5-1/2 minutes	10,000 to >600,000/mg	N/A

*Varies depending on tissue types

Nuclei In 5-1/2 Minutes

Reproducibly generate high-quality suspensions of nuclei from a wide range of tissues.

Merged DAPI-stained and bright-field images of small intestine, brain and heart tissue nuclei; DAPI stained liver nuclei. Courtesy of Dr. Minoda, Laboratory for Cellular Epigenomics, RIKEN Yokohama, Japan.

High Yields From Small Samples

Consistent High Yields of Nuclei

See It In Action

Watch this video to learn how to prepare single-nuclei suspension from mouse kidney tissue on the Singulator 100 System.

Single Cells in 20-60 Minutes

Reproducibly generate suspensions of single cells in high yield with high-viability from a wide range of tissues.

Bright-field images of mouse kidney and spleen tissue cells.

High Cell Yields

High Cell Viabilities

Tissues from different species demonstrated on the Singulator 100

Intuitive Software. Customizable Protocols.

Choose from a selection of pre-set protocols and pre-formulated reagents. Create your own protocols with customizable parameters, including mincing, enzyme incubation time, temperature, mixing and mechanical disruption profile. Optionally, use your own reagents.

Select from lists of Standard, Favorites, Recently Run protocols, or create your own protocol with customizable parameters.

Enter optional user notes and press RUN.

Step-by-step instructions and videos guide you through the system operation.

The internal camera and progress bar allow you to monitor tissue dissociation in real time.

Performance At A Glance

The Singulator 100^TM System comes with a unique Single-Shot Mechanism^TM for convenient cell-isolation reagent loading and a Reagent Chiller that stores reagents for up to 100 nuclei runs.

Singulator 100(TM) photo with features called out

High Yields &
Viability

Typical Yield:
• 10,000 to >500,000 cells/mg*
• 70 – 95% viability
• 20,000 to >600,000 nuclei/mg*
*tissue dependent

Reproducible
Results

• Consistent results, from
researcher to researcher
and lab to lab
• Improve success rates for
precious samples
• Minimize transcriptome
changes
• Optionally, use your reagents for your specific tissues

Fast Processing