Single Cells or Nuclei from Solid Tissues in Minutes
![S2 Genomics Singulator 100](https://s2genomics.com/wp-content/uploads/2021/03/SingulatorCompNewS2logo-500x608-v210305-247x300.png)
Explore the Singulator™ 100
Solid Tissue Dissociation. Automated.
The bench-top Singulator System and its single-use cartridges enable reproducible, rapid and hands-off tissue dissociations into single-cell or nuclei suspensions. Its ability to perform cold dissociation minimizes the expression of stress-related genes in cells and helps preserve RNA quality in nuclei. Researchers can now easily obtain suspensions of nuclei or high-viability cells for a wide range of single-cell analyses including the isolation of nuclei from FFPE samples.
Watch this video to learn more about the Singulator Platform.
Say Goodbye To Manual Tissue Dissociation.
From tissue to single cells or nuclei in minutes with walk-away operation.
Minimal Variability. Multiple Applications.
Ideal for genomics, cell biology and other ‘omics applications, including scRNA-Seq, snRNA-Seq, ATAC-Seq, CITE-Seq, FACS, and immuno-oncology. S2 Genomics provides a selection of pre-set protocols and pre-formulated reagents for cell isolations for an expanding set of mouse, rat, and human tissues, including tumors. Isolate nuclei from fresh, flash frozen, OCT, and FFPE tissues.
Fast. High Yield. High Viability.
Process fresh, frozen, OCT, and FFPE tissues.
TISSUE TYPE | Process Time | Yield* | Viability* |
|
---|---|---|---|---|
Cells | Fresh | 20-60 minutes | 10,000 to >500,000/mg | 70-95% |
Nuclei | Fresh, Frozen, OCT, FFPE | 7-12 minutes | 10,000 to >600,000/mg | N/A |
*Varies depending on tissue types
Nuclei is as Little as 7 Minutes
Reproducibly generate high-quality suspensions of nuclei from a wide range of tissues.
High Yields From Small Samples
![Figure-4-Nuclei-Recovered-from-Mouse-Tissues-640x414 Nuclei Recovered from Mouse Tissues](https://s2genomics.com/wp-content/uploads/2024/04/Figure-4-Nuclei-Recovered-from-Mouse-Tissues-640x414-1.png)
Consistent High Yields of Nuclei
![Fig3-Nuclei-Yield-per-mg-Sample-640x350 Nuclei Yield per mg Sample](https://s2genomics.com/wp-content/uploads/2024/04/Fig3-Nuclei-Yield-per-mg-Sample-640x350-1.png)
The Singulator Platform is Reproducible and Precise
The Singulator Platform isolates highly reproducible cell populations across technical replicates.
CLICK IMAGES FOR A LARGER VIEW
Integrated UMAP projection of six technical replicates isolated from a single mouse kidney dissociated with the Singulator 100 and 200 Platform. Nuclei were profiled with the 10x Genomics Chromium Next GEM Single Cell 3ʹ v3.1 solution. Nuclei were isolated from a single mouse kidney on two different Singulator 100 and both bays of two different Singulator 200 instruments.
UMAP colored by different cell types. The Singulator Platform was able to capture the representative cell types in the kidney, including rare, large and fragile cells like podocytes.
See It In Action
Watch this video to learn how to prepare single-nuclei suspension from mouse kidney tissue on the Singulator Platform.
Single Cells in 20-60 Minutes
Reproducibly generate suspensions of single cells in high yield with high-viability from a wide range of tissues.
Bright-field images of mouse kidney and spleen tissue cells.
High Cell Viabilities
![22](https://s2genomics.com/wp-content/uploads/2020/09/22-1.jpg)
High Cell Yields
![11](https://s2genomics.com/wp-content/uploads/2020/09/11-1.jpg)
Intuitive Software. Customizable Protocols.
Choose from a selection of pre-set protocols and pre-formulated reagents. Create your own protocols with customizable parameters, including mincing, enzyme incubation time, temperature, mixing and mechanical disruption profile. Optionally, use your own reagents.
Select from lists of Standard, Favorites, Recently Run protocols, or create your own protocol with customizable parameters.
Enter optional user notes and press RUN.
Step-by-step instructions and videos guide you through the system operation.
The internal camera and progress bar allow you to monitor tissue dissociation in real time.
Performance At A Glance
The Singulator 100TM System comes with a unique Single-Shot MechanismTM for convenient cell-isolation reagent loading and a Reagent Chiller that stores reagents for up to 100 nuclei runs.
High Yields &
Viability
Typical Yield:
• 10,000 to >500,000 cells/mg*
• 70 – 95% viability
• 20,000 to >600,000 nuclei/mg*
*tissue dependent
Reproducible
Results
• Consistent results, from
researcher to researcher
and lab to lab
• Improve success rates for
precious samples
• Minimize transcriptome
changes
• Optionally, use your reagents for your specific tissues
Fast Processing
• Nuclei in 7-12 minutes
• Single cells in 20-60 minutes
Simple Setup.
Walk-away Operation
• Load tissue and press RUN
in < 1 minute
• Intuitive touch-screen interface
• Minimal operator training