The Singulator™ Platform

Reproducible isolation of single cells or nuclei from solid tissue in minutes

Tissue Dissociation Optimized for Reproducibility

One bottleneck that has emerged in scRNA-seq is the limitation of manual sample preparation. Individual users can introduce bias in cell preparation, which may influence the ability to detect rare cell types and cause changes in gene expression.

The Singulator™ Platform is an easy-to-use, flexible, automated solution for the rapid dissociation of solid tissue into high-quality, viable single cells or intact nuclei compatible with numerous single-cell genomics applications.

The Singulator™ 200+

Isolates single nuclei in 6 minutes from as little as 2 mg of input tissue:
Deparaffinizes and dissociates FFPE samples in as little as 40 minutes with no manual intervention

Flexible, Automated Solution that Replaces Manual Tissue Processing

Reproducible and Precise

Produces consistent results, ensuring high-quality data. Isolates cell populations with minimal bias, essential for single-cell genomics applications. 

Automated

Streamlines the workflow, reducing manual steps, saving time and minimizing opportunities for mistakes. 

User-friendly

Intuitive interface and easy-to-follow protocols make it accessible to all users. 

Minimal Sample Loss

Designed to minimize sample loss, preserving valuable biological material.

Tissue Collection
Tissue Dissociation
Single Cell Partitioning and Barcoding
Library Prep
Next Gen Sequencing
Analysis

Singulator™ 100 System
Single sample processing

Singulator™ 200 System
Simultaneous sample processing

Singulator™ 200+ System
FFPE sample processing

Speed Up your Sample Prep

CARTRIDGECell IsolationLarge Cell IsolationNuclei IsolationNIC+FFPE
Cell IsolationLarge Cell IsolationNuclei IsolationNIC+FFPE
SUPPORTED TISSUE INPUTFreshFreshFresh, Frozen, OCTFresh, Frozen, OCT, FFPEFFPE
PROCESSING TIME20-60 mins20-60 mins6-12 mins6-12 mins40-80 mins
SUPPORTED RANGE
(mg OF INPUT TISSUE)
20-300 mg20-300 mg20-300 mg2-300 mg1 x 50 µm curl

Compatible with a Variety of Downstream Applications

Consumables

Cartridges

Designed for the rapid isolation of cells or nuclei from solid tissue

RNase Inhibitor

Preserve RNA integrity for downstream analysis

Sample Clean Up

Obtain cleaner samples with debris removal

Cell and Nuclei Isolation Reagents

Optimized to isolate cells or nuclei from solid tissue while preserving gene expression profiles

Request a quote or connect with one of our technical experts.

Still have questions? Please email inquiries@s2genomics.com.

Singulator™ Cartridges

The Singulator™ Cartridges are single-use consumables designed for rapid and efficient isolation of cells or nuclei from tissue samples. Compatible with the Singulator™ Platform and tailored for use with our specialized isolation reagents, the cartridges enable the extraction of cells or nuclei from samples sized 2-250 mg in as little as 6 minutes, ensuring high-yield, high-quality cell or nuclei suspensions for downstream genomic applications. The primary benefits include:

  1. Reproducible and Precise: The standardized disruption chamber and grinder combine with automated disruption to ensure consistent performance across different days and users. When used in combination with cell and nuclei isolation reagents, cartridges isolate consistent cell populations with minimal bias, essential for single-cell genomics applications.
  2. Versatile: Compatible with most of tissue types, enabling broad single-cell genomics applications.
  3. Efficient: Streamlines the workflow, reducing manual steps, saving time and minimizing opportunities for mistakes.
  4. Minimal Sample Loss: Designed to minimize sample loss, preserving valuable biological material.

These benefits collectively help in achieving high-quality, reliable data from precious samples, aligning with the Singulator™ Platform’s strengths in reproducibility and precision.

Ordering Information

RNAse Inhibitor V2

RNase Inhibitor V2 has been optimized to inhibit endogenous RNase activity during nuclei isolation, thereby ensuring the integrity and stability of RNA within single nuclei for gene expression detection. The primary benefits include:

  1. Compatible: RNase Inhibitor V2 was designed for use with Singulator™ Nuclei Isolation Kits, sold in sizes that cover both isolation and post-processing needs.
  2. Versatile: Robust protection against RNases A, B, and C during nuclei isolation, ensuring RNA integrity with its high stability and maximum inhibition. Compatible with a most of tissue types, enabling broad single-cell genomics applications.
  3. Enhanced Activity: RNase Inhibitor V2 demonstrates enhanced activity over its predecessor, V1.
  4. Scalable: Suitable for small-scale experiments and large-scale studies. RNase Inhibitor V2 makes automated single-nuclei isolation affordable without sacrificing quality.

These benefits collectively help in achieving high-quality, reliable data from precious samples, aligning with the Singulator™ Platform’s strengths in reproducibility and precision.

Ordering Information

Sample Clean Up and Preparation for 10x Assay Loading

These reagents are designed to prepare nuclei isolated with the Singulator™ Platform for loading into 10x Genomics Chromium Gene Expression assays by effectively removing unwanted debris or adjusting the concentration of magnesium. The primary benefits include:

  1. Reproducible and Precise: Consistent removal of debris and addition of magnesium ensures more uniform sample preparations across different runs and experiments, contributing to reproducible and precise results.
  2. Improved Sample Quality: By reducing debris, the reagent increases the purity of the isolated nuclei, leading to more accurate and reliable downstream analyses.
  3. Reduced Background Noise: Lower levels of debris can minimize background in assays, improving signal clarity and sensitivity.
  4. Compatible: Seamlessly integrates with downstream single-cell genomics applications.

These benefits collectively help in achieving high-quality, reliable data from precious samples, aligning with the Singulator™ Platform’s strengths in reproducibility and precision.

Ordering Information

Cell and Nuclei Isolation Reagents

The Cell and Nuclei Isolation Reagents for the Singulator™ Platform are optimized for efficient and reproducible isolation of cells or nuclei from solid tissue when used in combination with the Singulator™ instrument and cartridges. The primary benefits include:

  1. Reproducible and Precise: Standardized reagents ensure consistent performance across different days and users. When used in combination with the Singulator™ cartridges, the isolation reagents isolate consistent cell populations with minimal bias, essential for single-cell genomics applications.
  2. Versatile: Compatible with most of tissue types, enabling broad single-cell genomics applications.
  3. Efficient and Easy to Use: The reagents are provided in ready-to-use formulations, simplifying the preparation process.
  4. Scalable: Suitable for small-scale experiments and large-scale studies.

These benefits collectively help in achieving high-quality, reliable data from precious samples, aligning with the Singulator™ Platform’s strengths in reproducibility and precision.

Ordering Information