The Singulator™ Platform

The Singulator™ Cartridges are single-use consumables designed for rapid and efficient isolation of cells or nuclei from tissue samples. Compatible with the Singulator™ Platform and tailored for use with our specialized isolation reagents, the cartridges enable the extraction of cells or nuclei from samples sized 2-250 mg in as little as 6 minutes, ensuring high-yield, high-quality cell or nuclei suspensions for downstream genomic applications. The primary benefits include:

1. Reproducible and Precise: The standardized disruption chamber and grinder combine with automated disruption to ensure consistent performance across different days and users. When used in combination with cell and nuclei isolation reagents, cartridges isolate consistent cell populations with minimal bias, essential for single-cell genomics applications.
2. Versatile: Compatible with most of tissue types, enabling broad single-cell genomics applications.
3. Efficient: Streamlines the workflow, reducing manual steps, saving time and minimizing opportunities for mistakes.
4. Minimal Sample Loss: Designed to minimize sample loss, preserving valuable biological material.

These benefits collectively help in achieving high-quality, reliable data from precious samples, aligning with the Singulator™ Platform’s strengths in reproducibility and precision.

RNase Inhibitor V2 has been optimized to inhibit endogenous RNase activity during nuclei isolation, thereby ensuring the integrity and stability of RNA within single nuclei for gene expression detection. The primary benefits include:

1. Compatible: RNase Inhibitor V2 was designed for use with Singulator™ Nuclei Isolation Kits, sold in sizes that cover both isolation and post-processing needs.
2. Versatile: Robust protection against RNases A, B, and C during nuclei isolation, ensuring RNA integrity with its high stability and maximum inhibition. Compatible with a most of tissue types, enabling broad single-cell genomics applications.
3. Enhanced Activity: RNase Inhibitor V2 demonstrates enhanced activity over its predecessor, V1.
4. Scalable: Suitable for small-scale experiments and large-scale studies. RNase Inhibitor V2 makes automated single-nuclei isolation affordable without sacrificing quality.

These benefits collectively help in achieving high-quality, reliable data from precious samples, aligning with the Singulator™ Platform’s strengths in reproducibility and precision.

These reagents are designed to prepare nuclei isolated with the Singulator™ Platform for loading into 10x Genomics Chromium Gene Expression assays by effectively removing unwanted debris or adjusting the concentration of magnesium. The primary benefits include:

1. Reproducible and Precise: Consistent removal of debris and addition of magnesium ensures more uniform sample preparations across different runs and experiments, contributing to reproducible and precise results.
2. Improved Sample Quality: By reducing debris, the reagent increases the purity of the isolated nuclei, leading to more accurate and reliable downstream analyses.
3. Reduced Background Noise: Lower levels of debris can minimize background in assays, improving signal clarity and sensitivity.
4. Compatible: Seamlessly integrates with downstream single-cell genomics applications.

These benefits collectively help in achieving high-quality, reliable data from precious samples, aligning with the Singulator™ Platform’s strengths in reproducibility and precision.

The Cell and Nuclei Isolation Reagents for the Singulator™ Platform are optimized for efficient and reproducible isolation of cells or nuclei from solid tissue when used in combination with the Singulator™ instrument and cartridges. The primary benefits include:

1. Reproducible and Precise: Standardized reagents ensure consistent performance across different days and users. When used in combination with the Singulator™ cartridges, the isolation reagents isolate consistent cell populations with minimal bias, essential for single-cell genomics applications.
2. Versatile: Compatible with most of tissue types, enabling broad single-cell genomics applications.
3. Efficient and Easy to Use: The reagents are provided in ready-to-use formulations, simplifying the preparation process.
4. Scalable: Suitable for small-scale experiments and large-scale studies.

These benefits collectively help in achieving high-quality, reliable data from precious samples, aligning with the Singulator™ Platform’s strengths in reproducibility and precision.