Reproducible isolation of single cells or nuclei from solid tissue in minutes
One bottleneck that has emerged in scRNA-seq is the limitation of manual sample preparation. Individual users can introduce bias in cell preparation, which may influence the ability to detect rare cell types and cause changes in gene expression.
The Singulator™ Platform is an easy-to-use, flexible, automated solution for the rapid dissociation of solid tissue into high-quality, viable single cells or intact nuclei compatible with numerous single-cell genomics applications.
Isolates single nuclei in 6 minutes from as little as 2 mg of input tissue:
Deparaffinizes and dissociates FFPE samples in as little as 40 minutes with no manual intervention
Reproducible and Precise
Produces consistent results, ensuring high-quality data. Isolates cell populations with minimal bias, essential for single-cell genomics applications.
Automated
Streamlines the workflow, reducing manual steps, saving time and minimizing opportunities for mistakes.
User-friendly
Intuitive interface and easy-to-follow protocols make it accessible to all users.
Minimal Sample Loss
Designed to minimize sample loss, preserving valuable biological material.
Singulator™ 100 System
Single sample processing
Singulator™ 200 System
Simultaneous sample processing
Singulator™ 200+ System
FFPE sample processing
CARTRIDGE | Cell Isolation | Large Cell Isolation | Nuclei Isolation | NIC+ | FFPE |
---|---|---|---|---|---|
SUPPORTED TISSUE INPUT | Fresh | Fresh | Fresh, Frozen, OCT | Fresh, Frozen, OCT, FFPE | FFPE |
PROCESSING TIME | 20-60 mins | 20-60 mins | 6-12 mins | 6-12 mins | 40-80 mins |
SUPPORTED RANGE (mg OF INPUT TISSUE) | 20-300 mg | 20-300 mg | 20-300 mg | 2-300 mg | 1 x 50 µm curl |
Still have questions? Please email inquiries@s2genomics.com.
All products are for research use only.
© 2024 S2 Genomics, Inc. All rights reserved.
The Singulator™ Cartridges are single-use consumables designed for rapid and efficient isolation of cells or nuclei from tissue samples. Compatible with the Singulator™ Platform and tailored for use with our specialized isolation reagents, the cartridges enable the extraction of cells or nuclei from samples sized 2-250 mg in as little as 6 minutes, ensuring high-yield, high-quality cell or nuclei suspensions for downstream genomic applications. The primary benefits include:
These benefits collectively help in achieving high-quality, reliable data from precious samples, aligning with the Singulator™ Platform’s strengths in reproducibility and precision.
Part Number | Item Description |
---|---|
100-288-798 | Nuclei Isolation Bundle (24 samples) |
100-288-807 | Nuclei Isolation Bundle with RNase Inhibitor V2 (24 samples) |
100-289-043 | NIC+ Isolation Bundle (24 samples) |
100-289-152 | NIC+ Isolation Bundle with RNase Inhibitor V2 (24 samples) |
100-289-261 | Cell Isolation Bundle (24 samples) |
100-289-370 | Heart Cell Isolation Bundle (24 samples) |
100-284-221 | FFPE Nuclei Isolation Bundle with RNase Inhibitor V2 (24 samples) |
RNase Inhibitor V2 has been optimized to inhibit endogenous RNase activity during nuclei isolation, thereby ensuring the integrity and stability of RNA within single nuclei for gene expression detection. The primary benefits include:
These benefits collectively help in achieving high-quality, reliable data from precious samples, aligning with the Singulator™ Platform’s strengths in reproducibility and precision.
Part Number | Item Description |
---|---|
100-288-916 | RNase Inhibitor V2, 8 samples |
100-291-086 | RNase Inhibitor V2, 24 samples |
100-291-195 | RNase Inhibitor V2, 96 samples |
100-288-807 | Nuclei Isolation Bundle with RNase Inhibitor V2 (24 samples) |
100-289-152 | NIC+ Isolation Bundle with RNase Inhibitor V2 (24 samples) |
100-284-221 | FFPE Nuclei Isolation Bundle and RNase Inhibitor (24 samples) |
These reagents are designed to prepare nuclei isolated with the Singulator™ Platform for loading into 10x Genomics Chromium Gene Expression assays by effectively removing unwanted debris or adjusting the concentration of magnesium. The primary benefits include:
These benefits collectively help in achieving high-quality, reliable data from precious samples, aligning with the Singulator™ Platform’s strengths in reproducibility and precision.
Part Number | Item Description |
---|---|
100-253-628 | Nuclei Debris Removal Stock Reagent, 30 mL |
100-257-006 | Nuclei Loading Buffer (3 mM Magnesium), 30 mL |
The Cell and Nuclei Isolation Reagents for the Singulator™ Platform are optimized for efficient and reproducible isolation of cells or nuclei from solid tissue when used in combination with the Singulator™ instrument and cartridges. The primary benefits include:
These benefits collectively help in achieving high-quality, reliable data from precious samples, aligning with the Singulator™ Platform’s strengths in reproducibility and precision.
Part Number | Item Description |
---|---|
100-187-239 | Nuclei Reagent Pack (24 samples) |
100-187-348 | Nuclei Reagent Pack (96 samples) |
100-253-955 | Brain Reagent, 20 mL |
100-064-413 | Lung Reagent, 20 mL |
100-064-522 | Liver Reagent, 20 mL |
100-064-631 | Kidney Reagent, 20 mL |
100-064-740 | Spleen Reagent, 20 mL |
100-064-849 | Intestine Reagent, 20 mL |
100-253-846 | Heart Reagent, 20 mL |
100-254-082 | Skin Reagent, 20 mL |
100-253-955 | Tumor Reagent, 20 mL |