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A head-to-head evaluation of single cell RNA-seq technologies was performed between a droplet- based microfluidics approach (10x Genomics™ Chromium Next GEM Single Cell 3’Kit v3.1) and a combinatorial barcoding solution (Parse Biosciences Evercode WT v2). Cells from the kidney, an organ containing a complex mix of epithelial, endothelial, immune, and interstitial cell types, were chosen for an in-depth evaluation of cell type resolution and ambient RNA contamination.