Application Note: Single Nuclei Sequencing Using the
Singulator™ Platform and S2 Genomics
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The Singulator™ 100 and 200 can quickly isolate nuclei from a wide variety of tissue types, and from samples that are fresh, frozen, or OCT preserved. FFPE samples can be dissociated if previously deparaffinized and rehydrated. If the nuclei are intended for downstream single nuclei RNA Seq analysis, it is critical to prevent degradation of the RNA by endogenous RNase enzymes. To help preserve RNA integrity, RNase inhibitor is commonly added to samples during the isolation and purification of nuclei. Here we describe the use of S2 Genomics’ RNase Inhibitor combined with the Singulator for nuclei isolation for the purpose of single nuclei RNA sequencing applications.