Application & Tech Notes
TITLE
Single Nuclei Sequencing Using the Singulator™ Platform and S2 Genomics RNase Inhibitor
ABSTRACT
The Singulator™ 100 and 200 can quickly isolate nuclei from a wide variety of tissue types, and from samples that are fresh, frozen, or OCT preserved. FFPE samples can be dissociated if previously deparaffinized and rehydrated. If the nuclei are intended for downstream single nuclei RNA Seq analysis, it is critical to prevent degradation of the RNA by endogenous RNase enzymes. To help preserve RNA integrity, RNase inhibitor is commonly added to samples during the isolation and purification of nuclei. Here we describe the use of S2 Genomics’ RNase Inhibitor combined with the Singulator for nuclei isolation for the purpose of single nuclei RNA sequencing applications.
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Use of NIC+ with the Singulator™ 100 For Sub-20 mg Samples
ABSTRACT
The Singulator™ 100 can be used to quickly and efficiently isolate nuclei from a wide variety of tissue types, including samples that are fresh, frozen, or OCT preserved. It is ideal for genomics, cell biology and other ‘omics applications, including scRNA-Seq, snRNA-Seq, ATAC-Seq, CITE-Seq, FACS, and immuno-oncology. S2 Genomics provides a selection of customizable pre-set protocols and pre-formulated reagents for nuclei isolations from an expanding set of mouse, rat, and human tissues, including tumors. The new NIC+™ sample cartridge for nuclei isolation extends the range of sample sizes compatible with dissociation on the Singulator down to 1 mg. Here we describe the performance of the NIC+ cartridges and the Singulator 100 for nuclei isolations from samples below 20 mg in size.
TITLE
Use of RNase Inhibitor with the Singulator™ 100
ABSTRACT
The Singulator™ 100 can be used to quickly and efficiently isolate nuclei from a wide variety of tissue types, and from samples that are fresh, frozen, or OCT preserved. If the nuclei are intended for downstream RNA analysis, it is critical that care be taken to prevent degradation of the RNA in the nuclei by the activity of endogenous RNase enzymes, most importantly RNase A. To help preserve RNA integrity, RNAse inhibitor is commonly added to samples during the isolation and purification of nuclei. Here we describe the use of RNase inhibitors with the Singulator for nuclei isolations.
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Parse Biosciences | Comparison of Evercode™ WT v2 and Chromium™ Next GEM Single Cell 3’ Kit v3.1 in Mouse Kidney Cells
ABSTRACT
A head-to-head evaluation of single cell RNA-seq technologies was performed between a droplet- based microfluidics approach (10x Genomics™ Chromium Next GEM Single Cell 3’Kit v3.1) and a combinatorial barcoding solution (Parse Biosciences Evercode WT v2). Cells from the kidney, an organ containing a complex mix of epithelial, endothelial, immune, and interstitial cell types, were chosen for an in-depth evaluation of cell type resolution and ambient RNA contamination.
TITLE
Singulator 100™ and MARS®: Efficient Nuclei Isolation from a variety of tissue samples
ABSTRACT
MARS® acoustic technology utilizes active-microfluidics acoustics for the separation of single cells or nuclei without labeling, based only on the difference in their physical parameters. The cells are isolated with high recovery and ready for single-cell genomics.
The Singulator™ 100 System automates the processing of solid tissue samples into suspensions of single cells or nuclei with high yields and from small samples for a wide range of single-cell biology and genomic analyses.
