Use of NIC+ with the Singulator™ 100 For Sub-20 mg Samples
The Singulator™ 100 can be used to quickly and efficiently isolate nuclei from a wide variety of tissue types, including samples that are fresh, frozen, or OCT preserved. It is ideal for genomics, cell biology and other ‘omics applications, including scRNA-Seq, snRNA-Seq, ATAC-Seq, CITE-Seq, FACS, and immuno-oncology. S2 Genomics provides a selection of customizable pre-set protocols and pre-formulated reagents for nuclei isolations from an expanding set of mouse, rat, and human tissues, including tumors. The new NIC+™ sample cartridge for nuclei isolation extends the range of sample sizes compatible with dissociation on the Singulator down to 1 mg. Here we describe the performance of the NIC+ cartridges and the Singulator 100 for nuclei isolations from samples below 20 mg in size.
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