Tech Note: Sequencing Lung and Kidney Nuclei: Leveraging the Singulator™ 100 and Parse Biosciences Evercode™ Whole Transcriptome v2 Platform

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Advancements in high-throughput sequencing technologies have revolutionized our understanding of cellular heterogeneity and gene expression patterns. To comprehensively investigate the transcriptional landscapes of specific cell types within complex tissues, researchers have turned to single nucleus RNA sequencing (snRNA-seq) techniques. In this technical note, we demonstrate the compatibility of Singulator produced kidney and lung nuclei with the application of the Parse Biosciences single-cell whole transcriptome technology. The utilization of multiple platforms for single nuclei sequencing is crucial as it allows researchers to overcome the limitations and biases associated with individual technologies. The ability to both fix and run multiple samples simultaneously simplifies the single nuclei sequencing workflow, and combined with the ease of the Singulator system facilitates a wider range of sequencing applications.