Singulator™ Community

Your place to share protocols and best practices for the isolation of cells and nuclei from tissue.

To submit your protocol, download the appropriate template (fillable pdf), complete it by entering the protocol details and email the completed template to We’ll review it to ensure consistency with our guidelines, and will post it here for others to view and download.

If you have any questions, please contact us at

Nuclei Protocols

Warren Lab | University of Missouri

Chicken Thymus Nuclei Isolation

Institute of Experimental Botany of the Czech Academy of Sciences

Immature Embryos of Wild Sorghum Purpureosericeu Nuclei Isolation

University of Missouri

Pig Endometrium Nuclei Isolation