Integrated Sample Preparation Pipeline for scRNA-Seq and snRNA-Seq Utilizing the S2 Genomics Singulator™ Tissue Dissociation Platform and Parse Biosciences Evercode™ Whole Transcriptome Assay
Download Our Poster Presented at the Cell Symposia 2023
S2 Genomics has developed the Singulator™ 100 and 200 Systems to automate the isolation of singulated cells or nuclei from fresh, frozen, or OCT samples using disposable cartridges, reagents, and customizable protocols. The systems have been applied to prepare cells or nuclei from a wide range of tissues and organisms, including human, mouse, rat, chicken, pigs, insects, snails, zebrafish, Drosophila, honeybee, planaria, and plants.
Parse Biosciences has developed combinatorial barcoding technology that enables scalable single cell analyses without the limitations of microfluidic droplet approaches. With the Parse Evercode technology, it is possible to profile 10,000 to 1 million cells in a single experiment.
Single-cell sequencing workflows for multiple applications require reproducible generation of high-quality single-cell or nuclei suspensions, while combinatorial approaches to library preparation can, in turn, provide superior data quality to droplet-based methods. Here, we demonstrate the utility of combining automated dissociation of solid tissue samples with combinatorial library preparation and show results for a variety of tissue types, including snRNA-Seq data for adult mouse lung and kidney, and scRNA-Seq data for adult mouse kidney and skin.
This poster was presented at the Cell Symposia 2023 – The conceptual power of single cell biology.
