RNase Inhibitor
Preserve RNA Integrity for your Downstream Analyses
The Need for Integrity
When isolating nuclei, one or more types of RNase enzymes may be released which can cause the rapid degradation of nuclear RNA. To preserve RNA quality and ensure the success of cDNA preparations for downstream snRNA-Seq analyses, an RNase inhibitor is commonly added to a sample during the nuclei isolation and purification steps.
Our RNase Inhibitor
- Specially formulated to work with our Singulator automated tissue dissociation platform.
- Provided at a 40x working concentration.
- Comes in convenient packages sized for 2, 8 or 24 samples.
Product Availability
| Part Number | Package Size | Volume | US Price |
|---|---|---|---|
| 100-255-535 | 2-sample | 200 µL | Request a Quote |
| 100-255-644 | 8-sample | 800 µL | Request a Quote |
| 100-256-761 | 24-sample | 3x 800 µL vials | Request a Quote |
FREE RESOURCE
Tech Note: Use of RNase Inhibitor with the Singulator™ 100
The Singulator™ 100 can be used to quickly and efficiently isolate nuclei from a wide variety of tissue types, and from samples that are fresh, frozen, or OCT preserved. If the nuclei are intended for downstream RNA analysis, it is critical that care be taken to prevent degradation of the RNA in the nuclei by the activity of endogenous RNase enzymes, most importantly RNase A. To help preserve RNA integrity, RNAse inhibitor is commonly added to samples during the isolation and purification of nuclei. Here we describe the use of RNase inhibitors with the Singulator for nuclei isolations.
Resources
Poster Download: Nuclei Isolation from Immature Embryos of Wild Sorghum purpureosericeum with the Singulator™ 100 system
By Brad Oliver
/ February 14, 2023
Poster Download: Automated processing of solid tissues into single cells or nuclei for genomics and cell biology applications with the Singulator™ 100 and 200 systems
By Yolanda Fintshenko
/ June 23, 2022
Download Poster
Poster Download: Automated processing of solid tissues into single cells or nuclei with the Singulator™ 100 system: scRNA-Seq, and ATAC-Seq data on Human and Mouse Tissues – AGBT 2022
By Yolanda Fintshenko
/ June 22, 2022
Download Poster
Tech Note: Use of NIC+ with the Singulator 100 For Sub-20 mg Samples
By S2 Genomics
/ June 3, 2022
Download Tech Note
Tech Note: Singulator™ 100 and MARS®: Efficient Nuclei Isolation from a variety of tissue samples
By Brad Oliver
/ May 23, 2022
Download Tech Note
Tech Note: Use of RNase Inhibitor with the Singulator™ 100
By Brad Oliver
/ May 23, 2022
Download Tech Note
Keystone 2021 Poster Download: Automated Processing of Solid Tissues into Single Cells or Nuclei for Single Cell Analysis
By S2 Genomics
/ March 19, 2021
Download Poster
Automated Nuclei Isolation From Tissues – Singulator 100 Data Sheet
By admin
/ May 5, 2020
Download Data Sheet
Poster Download: Automated Processing of Solid Tissues into Single Cells or Nuclei for Sequencing
By admin
/ March 5, 2020
Download Poster
